Coding

Part:BBa_K4632018:Design

Designed by: Haolong Lai   Group: iGEM23_SCAU-China   (2023-10-09)


LM35 (T222I)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 21
    Illegal PstI site found at 113
    Illegal PstI site found at 363
    Illegal PstI site found at 521
    Illegal PstI site found at 599
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 113
    Illegal PstI site found at 363
    Illegal PstI site found at 521
    Illegal PstI site found at 599
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 633
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 21
    Illegal PstI site found at 113
    Illegal PstI site found at 363
    Illegal PstI site found at 521
    Illegal PstI site found at 599
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 21
    Illegal PstI site found at 113
    Illegal PstI site found at 363
    Illegal PstI site found at 521
    Illegal PstI site found at 599
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

What have we done? (SCAU-China 2023)

We optimized the codons of the provided sequence for Escherichia coli expression. We successfully characterized its performance in E. coli TOP10. For more detailed characterization results, please refer to our composite_part-BBa_K4632024[1]

Source

Jiang W, He X, Luo Y et al. Two Completely Orthogonal Quorum Sensing Systems with Self-Produced Autoinducers Enable Automatic Delayed Cascade Control[J]. ACS SYNTH The text sequence was generously provided by the corresponding author of the original paper, Professor Liang Quanfeng. Subsequently, we ordered its synthesis from GUANGZHOU IGE BIOTECHNOLOGY LTD.

Contact Information: liangquanfeng@sdu.edu.cn

References

Jiang W, He X, Luo Y et al. Two Completely Orthogonal Quorum Sensing Systems with Self-Produced Autoinducers Enable Automatic Delayed Cascade Control[J]. ACS SYNTH BIOL, 2020,9(9):2588-2599.